Single-molecule fluorescence provides high resolution spatial distributions of ribosomes and RNA polymerase (RNAP) in live, rapidly growing E.coli.Ribosomes are more strongly segregated from the nucleoids (chromosomal DNA) than previous widefield fluorescence studies suggested.While most transcription may be co-translational, the evidence indicates
Robotic Pectoral Fin Thrust Vectoring Using Weighted Gait Combinations
A method was devised to vector propulsion of a robotic pectoral fin by means of actively controlling fin surface curvature.Separate flapping Soap fin gaits were designed to maximize thrust for each of three different thrust vectors: forward, reverse, and lift.By using weighted combinations of these three pre-determined main gaits, new intermediate
Biguanides Induce Acute de novo Lipogenesis in Human Primary Sebocytes
James Nicoll, Benjamin M Buehrer Zen-Bio, Inc., Research Triangle Park, NC, USACorrespondence: Benjamin Shop Service M BuehrerZen-Bio, Inc., 3200 East Highway 54, Suite 100, PO Box 13888, Research Triangle Park, NC 27709, USATel +1 919 547 0692Fax +1 919 547 0693Email [email protected]: Acne arises during puberty, in part, due to elevated
Bacterial co-infection and antibiotic stewardship in patients with COVID-19: a systematic review and meta-analysis
Abstract Introduction Understanding the proportion of patients with COVID-19 who have respiratory bacterial co-infections and the responsible pathogens is important for managing COVID-19 effectively while ensuring responsible antibiotic use.Objective To estimate the frequency of bacterial co-infection in COVID-19 hospitalized patients and of antibi
Twisting and swiveling domain motions in Cas9 to recognize target DNA duplexes, make double-strand breaks, and release cleaved duplexes
The CRISPR-associated protein 9 (Cas9) has been engineered as a precise gene editing tool to make double-strand breaks.CRISPR-associated protein 9 binds the folded guide RNA (gRNA) that serves as a binding scaffold to guide it to the target DNA duplex via a RecA-like strand-displacement mechanism but without ATP Bedding Set binding or hydrolysis.Th